The Ruthenium Red Test Biology Essay

The Ruthenium Red Test Biology Essay This test is subjected to identification of carbohydrates. 1 w/v dispersion of test substance was prepared in distilled water. Test mixture was combined with a small amount of molischs reagent in a test tube. After mixing, small amount of concentrated sulphuric acid was slowly added down the sides of the sloping test tube to obtain violet ring at junction. 5.2.1.4. Ninhydrin test This test is subjected to identification of proteins. 2 ml aqueous solution of test substance and 1 ml alcoholic ninhydrin solution was mixed in test tube and heated at 80Â ° C for 5 min to obtain red color. 5.2.1.5. Test with iodine This test is subjected to identification of non reducing polysachharides (starch). 1 % w/v dispersion of test substance was prepared in distilled water. 3 ml test solution and few drops of dilute Iodine solution were mixed to obtain blue color after cooling and no color after heating. 5.2.1.6. Biuret test This test is subjected to identification of proteins. 1 % w/v dispersion of test substance was prepared in distilled water. 3 ml test solution, 1 ml 4% sodium hydroxide and 2 drops of copper sulphate solution were mixed to obtain violet to pink. 5.2.1.7. Salkowski test This test is subjected to identification of steroids. 1 % w/v dispersion of test substance was prepared in distilled water. 2 ml test solution, 2 ml chloroform and 2 ml conc. sulphuric acid were mixed and shaken well to obtain red colored chloroform layer and greenish yellow fluorescence in acid layer. 5.2.1.8. Baljet test This test is subjected to identification of glycosides. 1 % w/v dispersion of test substance was prepared in distilled water. 2ml test solution and 2 ml sodium picrate was mixed in test tube to obtain yellow to orange color. 5.2.1.9. Shinoda test This test is subjected to identification of flavonoids. 1 % w/v dispersion of test substance was prepared in distilled water. 3 ml test substance, 5 ml 95% ethanol, 2 drops of Conc. HCl and 0.5 g magnesium turnings were mixed to obtain pink color. 5.2.1.10. Wagners test This test is subjected to identification of alkaloids. 1 % w/v dispersion of test substance was prepared in distilled water and filtered. 3 ml test solution filtrate was mixed with 2 drops of Wagners reagent to obtain reddish brown precipitate. 5.2.1.11. Test with acetic acid solution This test is subjected to identification of alkaloids. 1 % w/v dispersion of test substance was prepared in distilled water. 2 ml test solution and acetic acid solution were mixed to obtain red color. 5.2.2. Physicochemical studies123 5.2.2.1. Viscosity 1% w/v solution of test substance was prepared in distilled water and 0.1 N HCl separately. Solutions were kept at 37 Â ± 2 Â °C for 6 hours. Viscosity of test solutions determined using Brookfield viscometer using spindle no. 3 at 50 rpm (Model No. DV-E). 5.2.2.2. pH determination124 1 % w/v dispersion of test substance in distilled water was prepared by shaking for 30 min and the pH determined using a pH meter (Elico). 5.2.2.3. Swelling Index88 Swelling index is the volume in ml occupied by 1 g of a drug, including any adhering mucilage, after it has swollen in an aqueous liquid for 4 hours. 1 g test substance (Fine powder) was placed in 25 ml ground glass stoppered measuring cylinder with graduations in 0.5 ml divisions. The test substance was moistened with 1.0 ml of 96% ethanol and 25 ml distilled water was added. Cylinder was closed using stopper. Cylinder was shaken every 10 min time interval for 1 hour and then was allowed to stand for 3 hours. Volume occupied by the test sample was determined in ml after decanting the supernatant clear liquid. Swelling index was calculated from average of three tests. Same experiment was performed using 25 ml 0.1 N hydrochloric acid instead of distilled water. Swelling index was calculated using following equation. Vt = Volume occupied by test substance after hydration, at time 3 hrs. V0 = Volume occupied by test substance before hydration, at time zero. 5.2.2.4. Bulk density123 Bulk Density was determined using Borosil 50 ml measuring cylinder. The volume of 5 gm mucilage powder was noted. Value of Bulk density was calculated by, 5.2.2.5. Tapped density123 Tapped Density was determined using Borosil 50 ml measuring cylinder. The volume of known weight 5gm was noted. The cylinder was given 250 taps on using tapped density apparatus. Value of Tapped density was calculated by 5.2.2.6. Carrs compressibility index123 Carrs compressibility index of AER mucilage powder was calculated from respective tapped density and bulk density by, 5.2.2.7. Loss on drying88 In flat bottom dish, 50 mm in diameter and 30 mm in height, 1 g fine powdered test substance dried in oven at 100-105 C for 3 hours. The test substance was allowed to cool in desiccator over diphosphorus pentoxide for 24 hours and weighed. Percentage loss on drying was calculated by following equation. Weight of empty dish = A g Weight of test substance taken = Y g Weight of dish containing test substance after drying and placing in desiccator = B g Weight of test substance after drying = (B A) g

The Identity of Thomas Pynchon :: Biography Biographies Essays

The Identity of Thomas Pynchon The identity of Thomas Pynchon is as elusive as the sticky, complex webs of meaning woven into his prose. As America's most "famous" hidden author, Pynchon produces works which simultaneously deal with issues of disappearance and meaning, of identity and nothingness in a fashion that befuddles some and delights others. He speaks to the world from his invisible pulpit, hiding behind a curtain of anonymity that safely disguises his personality from the prying eyes of critics and fans alike. Without a public author presence, readers are forced to derive the identity of the author instead from the author's actual works. When searching for the identity of Pynchon, and indeed the notion of identity itself, the novels of Thomas Pynchon offer an interesting starting point. Questions of identity and meaning are shrouded beneath a veil of conspiracy in The Crying of Lot 49, Pynchon's second novel and his shortest. Throughout the novel there are snatches of hidden agendas and mysterious plans; it is a world run by Pierce Inverarity, a character who is dead when the novel opens yet remains an active presence throughout the work. This seems to fit Pynchon's situation rather nicely as the ghostly moderator of a tired world, leading his main character Oedipa Maas on a quest for meaning while blindly groping for clues about a conspiratorial mail system known only as the Trystero. Oedipa's quest echos the quest of everyone; she wishes for an identity that makes some sense within the framework of her world. Thomas Pynchon, by erasing himself from the public sphere, is questing for identity in his own right through his writings, letting Mrs. Maas do the searching for him. Little is known about Pynchon's life, and no one who knows him seems to be willing to add to the miniscule pile of information currently available about him. His most recent published photograph dates back to 1953. Beginning at the beginning, he was born on May 8, 1937 in Glen Cove, New York. He attended Cornell University and received a degree in English in 1959. He worked at Boeing Company in Seattle as a technical writer until his first novel V. appeared in 1963 (Gray 70). From that point onward, Pynchon vanished from the public eye. Information about any part of Pynchon's life after V. The Identity of Thomas Pynchon :: Biography Biographies Essays The Identity of Thomas Pynchon The identity of Thomas Pynchon is as elusive as the sticky, complex webs of meaning woven into his prose. As America's most "famous" hidden author, Pynchon produces works which simultaneously deal with issues of disappearance and meaning, of identity and nothingness in a fashion that befuddles some and delights others. He speaks to the world from his invisible pulpit, hiding behind a curtain of anonymity that safely disguises his personality from the prying eyes of critics and fans alike. Without a public author presence, readers are forced to derive the identity of the author instead from the author's actual works. When searching for the identity of Pynchon, and indeed the notion of identity itself, the novels of Thomas Pynchon offer an interesting starting point. Questions of identity and meaning are shrouded beneath a veil of conspiracy in The Crying of Lot 49, Pynchon's second novel and his shortest. Throughout the novel there are snatches of hidden agendas and mysterious plans; it is a world run by Pierce Inverarity, a character who is dead when the novel opens yet remains an active presence throughout the work. This seems to fit Pynchon's situation rather nicely as the ghostly moderator of a tired world, leading his main character Oedipa Maas on a quest for meaning while blindly groping for clues about a conspiratorial mail system known only as the Trystero. Oedipa's quest echos the quest of everyone; she wishes for an identity that makes some sense within the framework of her world. Thomas Pynchon, by erasing himself from the public sphere, is questing for identity in his own right through his writings, letting Mrs. Maas do the searching for him. Little is known about Pynchon's life, and no one who knows him seems to be willing to add to the miniscule pile of information currently available about him. His most recent published photograph dates back to 1953. Beginning at the beginning, he was born on May 8, 1937 in Glen Cove, New York. He attended Cornell University and received a degree in English in 1959. He worked at Boeing Company in Seattle as a technical writer until his first novel V. appeared in 1963 (Gray 70). From that point onward, Pynchon vanished from the public eye. Information about any part of Pynchon's life after V.

Why Cosmetology

I haven't had an actual job related to this career, other than doing freelance Jobs when asked to. It is because of the freelance work Vive done that I decided to take this career path for now. I thought to myself, why not make more money off something I do already anyway? Like Jessica, from Real Stories, I have changed my mind a lot about the kind of career I want to. One thing I am sure of now though Is that I don't want to be a cosmetologist for the rest of my life.I can definitely see myself In a Job that Is unrelated to Cosmetology. My goal Is to make a change in people's lives, and I feel Like this Is one way of doing that. If making people feel better about their appearance gives them self-confidence, I have done my lob. This relates to the career I actually want which Is to be a Youth Probation Officer because I want to make a change In young people at an early age. A cosmetologist and a probation officer are two completely different careers, but when you really hint about it they both try and make an impact in people's lives.I am sometimes concerned that I will go through what Jessica from Real Stories went through. I am scared that the career I want is nothing like what I expected. If I were Jessica I would have done more research on the work environment of the job. Online research is always good, but there is nothing like interviewing someone that is exactly where you want to be. They have been there and can tell you exactly what to expect. We should always have options in life.I personally get bored of the same routine. This could affect my decision of the career I want in the long run. So, I think it is a good idea to attend graduate school to expand our options. We never know how a job really is until we actually start working it. It doesn't matter the amount of research we do on a specific career because everyone's experience is different. We are individuals, and some might love it and others might hate it. We should always have options and be pr epared.

Comparing Updikes AP and Joyces Araby Essay - 1336 Words

Comparing Updikes A P and Joyces Araby John Updikes A P and James Joyces Araby share many of the same literary traits. The primary focus of the two stories revolves around a young man who is compelled to decipher the difference between cruel reality and the fantasies of romance that play in his head. That the man does, indeed, discover the difference is what sets him off into emotional collapse. One of the main similarities between the two stories is the fact that the main character, who is also the protagonist, has built up incredible, yet unrealistic, expectations of women, having focused upon one in particular towards which he places all his unrequited affection. The expectation these men hold when finally face†¦show more content†¦Both stories delve into the unstable psyche of a young man who is faced with one of lifes most difficult lessons: that things are not always as they appear to be. Telling the tale as a way of looking back on his life, the protagonist allows the reader to follow his lifes lessons as they are learned, imparting upon the audience all the emotional pain and suffering endured for each one. The primary focal point is the young mans love for a completely unattainable girl who unknowingly riles the man into such a sexual and emotional frenzy that he begins to confuse sexual impulses for those of honor and chivalry (Wells, 1993, p. 127). It is this very situation of self-deception upon which both stories concentrate that brings the young man to his emotional knees as he is forced to compensate for the emptiness and longing in the young boys life (Norris 309). As much as Updikes rendition is different from Joyces original work, the two pieces are as closely related as any literary writings can be. Specifically addressing details, it can be argued that Updike missed no opportunity to fashion A P as much after Araby as possible. For example, one aspect of womanhood that fascinates and intrigues both young men is the whiteness of the girls skin. This explicit detail is n ot to be taken lightly in either piece, for the implication is integral to the other important story elements, particularly as they deal with female obsession. FocusingShow MoreRelatedThe Stages of Maturation in James Joyce’s Araby John Updikes AP from the Authors Perspective902 Words   |  4 PagesWhen comparing the views of both James Joyce and John Updike on maturation from adolescence to adulthood it will be important to continually compare two of their similar works in Joyce’s â€Å"Araby† and Updike’s â€Å"AP†. James Joyce and John Updike follow similar views with the latter using Joyce as a foundation and following in similar footsteps; both authors follow a process of maturation based on the allure of love, while doing it at different stages of each of the protagonists’ lives resulting in similarRead MoreHow Cultural Expectations Affect Both Men And Women1264 Words   |  6 PagesDivergent series’ respectively, some autho rs take a more realistic approach. James Joyce’s unnamed narrator in his short story Araby and John Updike’s Sammy from AP are both affected by the gender roles in an unorthodox way that shows how cultural expectations affect both men and women. Both characters used the opposite sex as an escape from their current realities, but are disappointed in the end. In AP, John Updike’s character experiences firsthand the negative effect of society’s expectations of